Chelator-induced inhibition of copper metalloenzymes in denitrifying bacteria
نویسندگان
چکیده
منابع مشابه
Accumulation of nitrogen oxides in copper-limited cultures of denitrifying bacteria
Three strains of heterotrophic denitrifying bacteria were cultured in artificial seawater medium under trace metal clean conditions to investigate their physiological response to changes in copper concentration. Decreasing the copper concentration in cultures of Pseudomonas stutzeri and Paracoccus denitrificans resulted in accumulation of nitrous oxide (N2O) gas compared to copper-replete cultu...
متن کاملAccumulation of Nitrogen Oxides in Copper-Limited Cultures of Denitrifying Bacteria
Three strains of heterotrophic denitrifying bacteria were cultured in artificial seawater medium under trace metal clean conditions to investigate their physiological response to changes in copper concentration. Decreasing the copper concentration in cultures of Pseudornonas stutzeri and Paracoccus denitrificans resulted in accumulation of nitrous oxide (N,O) gas compared to copper-replete cult...
متن کاملLocalization of the cytochrome cd1 and copper nitrite reductases in denitrifying bacteria.
The locations of cytochrome cd1 nitrite reductases in Pseudomonas aeruginosa and Pseudomonas fluorescens and copper nitrite reductases in Achromobacter cycloclastes and Achromobacter xylosoxidans were identified. Immunogold labeling with colloidal-gold probes showed that the nitrite reductases were synthesized exclusively in anaerobically grown (denitrifying) cells. Little immunogold label occu...
متن کاملDevelopment of three copper metalloenzymes in clover leaves.
Subterranean clover (Trifolium subterraneum L. cv Seaton Park) was grown in solution cultures containing adequate nitrogen both with and without Cu. After Cu deficiency had developed, Cu(2+) was added to some deficient plants and Cu content, protein content, and activities of three Cu metalloenzymes (diamine oxidase [EC1.4.3.6], ascorbate oxidase [EC1.10.3.3] and o-diphenol oxidase [EC1.10.3.1]...
متن کاملDetection and quantification of copper-denitrifying bacteria by quantitative competitive PCR.
We developed a quantitative competitive PCR (QC-PCR) system to detect and quantify copper-denitrifying bacteria in environmental samples. The primers were specific to copper-dependent nitrite reductase gene (nirK). We were able to detect about 200 copeis of nirK in the presence of abundant non-specific target DNA and about 1.2 x 10(3)Pseudomonas sp. G-179 cells from one gram of sterilized soil ...
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ژورنال
عنوان ژورنال: Limnology and Oceanography
سال: 2012
ISSN: 0024-3590
DOI: 10.4319/lo.2012.57.1.0272